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biology 0610printed 65 · PDF 65
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Aitchison College Academic
Enzymes in bean seeds are activated during germination. Some of these enzymes break
down protein stored in the seeds.
A large number of bean seeds were soaked and germinated. Researchers took samples of
germinating seeds over a period of 15 days. The seeds were chopped into small pieces and
crushed with water to make an extract. Equal quantities of the extracts were placed into
protein solutions at pH 5 and at pH 8.
The activity of the enzymes in each extract was determined by recording how quickly the
protein was broken down. The results are shown in Fig. 3.3.
40
+ pH 8
35
30
25 A
enzyme activity
/ arbitrary units
20
15 “4 saa pH5
10
0 2 8 10 12 14 16
time/ days
Fig. 3.3
(d) Describe the activity of the enzymes in the extracts at pH 5 over 15 days.
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